Chinese name D-fluorescein potassium salt 115144-35-9
English name D-Luciferinpotassiumsalt
CAS number 115144-35-9
Molecular formula C11H9KN2O3S2
Molecular weight 320.42
D-Luciferin is the substrate of firefly luciferase, and its quantum efficiency is 0.88, which is 20 times that of Luminol. D-Luciferin is a commonly used substrate for luciferase and is widely used in the entire field of biotechnology, especially in vivo imaging technology. Its mechanism of action is that under the action of ATP and luciferase, luciferin (substrate) can be oxidized and emit light. When there is an excess of luciferin, the number of light quanta produced is positively correlated with the concentration of luciferase. After the plasmid carrying the luciferase encoding gene (Luc) is transfected into cells, it is introduced into research animals such as rats and mice, and then luciferin is injected to detect changes in light intensity through bioluminescence imaging technology to monitor the development of the disease in real time. Or the therapeutic effect of drugs, etc. The influence of ATP on this reaction system can also be used to indicate energy or vital signs based on changes in bioluminescence intensity.